Photo-protection & mitochondria
To prevent sun-induced photo-aging and/or cutaneous carcinogenesis targeting mitochondrial function & network modulation represents a winning strategy.
The sun's wavelengths include UV, visible, and near-infrared (IR) wavelengths. Both UV and IR affect mitochondrial function by strongly increasing ROS production. Direct damages to DNA and proteins are also observed.
Our assays have been developed in several human primary cell models (fibroblasts, keratinocytes, etc.) and can be adapted to meet your specific needs.
These cell models, can further receive environmentally relevant doses of UV (A and B), IR or heat challenges to mimic sun exposure. We can follow mtDNA content to reflect the dynamics by which the mitochondrial network is adapting to this physical stress, the strategy for anti-oxidant defense, and the bioenergetic balance of the cells at different time points post-irradiation.
Which technologies to use
The Redox status package demonstrates anti-oxidant action following physical stress such as IR-, UV-, or heat-exposure. Anti-oxidation is one of the most prominent mechanisms to reduce damages to the cell following sun exposure.
This package includes the measurement of:
Cellular and mitochondrial radical oxygen species (ROS) production MnSOD and cSOD activities Total Gluthation activity Cellular catalase activity Lipid peroxidation level (TBARs) Protein carbonylation
The mitochondrial DNA content (mtDNA) is a highly relevant image analysis assay and a very good marker of efficacy against physical exposures such as UV, IR, or heat effect. It measures:
Mitochondrial DNA content Mitochondrial mass Mitochondrial biogenesis Nucleus integrity/morphology Cell viability
The Bioenergetic Balance Screen (BBS/BBS+) is a performant multiplexed assay developed to measure mitochondrial energy production and central metabolism in a same cell. If your active protects the cell from exposure-induced mtDNA depletion, it is also important to control that the protected DNA has not been damaged by the physical stress. The BBS provides the best readout of normal mitochondrial functioning, which can only occur if mtDNA has not been mutated.
The BBS is the multiplexed and integrated measurements, in a same cell, of:
Oxygen consumption ATP production Glycolysis level Cell viability TCA cycle turning (BBS+ only)