Mechanism of action

Why identify your drugs' mechanism(s) of action?

  • Early detection of a compound’s mechanism of action speeds its optimization at the pertinent target(s).
  • Better understanding of your drugs' mechanism of action helps refine your drugs' indication and define drugs' contingency plan.

All our assays are fully customizable and can be adapted to meet your specific needs. Contact us to learn how our technologies might be of value to you!

Which technologies to use

BBS + package

Our Bioenergetic Balance Screen (BBS+) helps you understand how your drug affects mitochondrial respiration and its interactions with central metabolism.

The BBS+ package is the multiplexed and integrated measurements, in a same cell, of:

  • Oxygen consumption
  • ATP production
  • Glycolysis level
  • TCA cycle turning
  • Cell viability

The BBS+ package is available in HTS/dose response studies using 4 different culture conditions (glucose-containing medium, galactose-containing medium, glutamine-containing medium, and glutamine-free medium).

Redox status

Our Redox balance suite, avalaible in HTS/dose response studies, is the measurement of (any or all):

  • ROS mitochondrial production
  • Cytoplasm ROS accumulation
  • MnSOD Cu/ZnSOD activities
  • Catalase activity
  • Total GSH activity
  • Lipid peroxidation level (TBARS)
  • Protein carbonylation
  • Cell viability

mtDNA content and quality control

Use our technologies to assess:

  • Changes in mitochondrial mtDNA content, which have been reported in a broad range of diseases and is a pertinent target in diabetes, obesity, cancer, and HIV complications.
  • Mitochondrial autophagy
    Mitophagy is the selective degradation of mitochondria by autophagy. ICDD provides image analyses to quantify this phenomenon.
  • Quantitative analysis of mitochondrial proteins or of proteins modulating mitochondrial functions.

Ask us about your targets of interest!