Slimming & toning

Slimming/toning & mitochondria

Caldeira da Silva and Cerqueira conclude in their article published in Aging Cell in 2008 that mild mitochondrial uncoupling is a highly effective anti-oxidant strategy mimicking calory restriction in healthy mammals. Calory restriction helps avoid the accumulation of fat and sculpt a firm body.

Control of fat mass can also be obtained by converting white adipocytes (WA) into brown-like adipocytes. The latter are specialized in adaptative thermogenesis and present differences from white adipocytes at the mitochondrial level along the following parameters:

  • Fatty acid oxidation enzymes
  • Respiratory chain components
  • Oxidative capacity
  • Expression of UCP-1

Selecting actives capable of modulating adaptative thermogenesis should provide a plausible and safe way to increase energy expenditure.

Mitochondria have also been associated to anti-cellulite activity. Indeed, smoothing out cellulite involves stimulating two key mechanisms: lipolysis in adipocytes (to deflate the adipocytes) and collagen synthesis in fibroblasts (to firm up the underlying tissue). Maintaining the mitochondria's physical and functionnal integrity is essential to stimulate original cell activity of lipolysis (adipocytes) and collagen synthesis (fibroblasts). These processes are also dependent on the cells' energy status (and thus mitochondria), especially for lipolysis, which can be inhibited by a decrease in ATP.

ICDD's solutions

Use the right cell model

We can test your active ingredients in human adipocytes precursors. This cell model is available to use with all of our technologies which target the pathways involved in the conversion of white to brown adipocytes.

Adult human primary fibroblasts are also available to identify your products' impact on these cells, responsible for collagen synthesis.

Which technology to use

Slimming & toning suite

The Slimming & toning suite combines functional assays from our BBS (bioenergetic balance screen) to measure mitochondrial respiration and fatty acid oxidation with quantitative western blot analysis to measure UCP-1 expression.

A simplified evaluation of mitochondrial physical and functionnal integrity can also be included, through a measure of cellular ROS (reactive oxygen species) production.