Slimming & toning
Slimming/toning & mitochondria
Caldeira da Silva and Cerqueira conclude in their article published in
Control of fat mass can also be obtained by converting white adipocytes (WA) into brown-like adipocytes. The latter are specialized in adaptative thermogenesis and present differences from white adipocytes at the mitochondrial level along the following parameters:
Fatty acid oxidation enzymes Respiratory chain components Oxidative capacity Expression of UCP-1
Selecting actives capable of modulating adaptative thermogenesis should provide a plausible and safe way to increase energy expenditure.
We can test your active ingredients in human adipocytes precursors. This cell model is available to use with all of our technologies which target the pathways involved in the conversion of white to brown adipocytes.
Adult human primary fibroblasts are also available to identify your products' impact on these cells, responsible for collagen synthesis.
Which technology to use
The Slimming & toning suite combines functional assays from our BBS (bioenergetic balance screen) to measure mitochondrial respiration and fatty acid oxidation with quantitative western blot analysis to measure UCP-1 expression.
A simplified evaluation of mitochondrial physical and functionnal integrity can also be included, through a measure of cellular ROS (reactive oxygen species) production.