Protection from urban pollutants

Urban pollutant's impact on mitochondria

An age-related impact

In addition to increasing incidence of respiratory diseases, pollution directly affects skin health. Indeed, it is responsible for increased dryness, premature aging, rashes, eczema, and acne. However, the mechanisms by which pollution reduces skin health are only partially understood. The leading hypothesis however include the generation of ROS by particles and skin aging due to mitochondrial damage.

When examining additional effects of pollutants on mitochondrial function, ICDD discovered that while both young and mature skins are affected by pollution, the impact is not the same for both.

ICDD has identified the impact of pollutants on:

  • Mitochondrial bioenergetics
  • Mitochondrial dynamics
  • Mitochondrial membrane permeability (strongest in mature skin)

In young skin however, the effects of pollution also ellicit compensatory mechanisms. On mature skin on the other hand, mitochondrial function has been impaired by aging and the cells' plasticity is reduced. As a result the pollutants' activity profile is more drastic, their impact even reaching mitochondrial membrane potential.

Interested in our results? Contact us for more details or call us to discuss what they might mean for you!

ICDD's solutions

Banking of human fibroblasts

ICDD banks human fibroblasts from donors of different ages and genders. Mitochondrial behavior signatures have been established to segregate age groups. Let us test your product's ability to protect from pollution-induced damage in your target population.

Which technologies to use

ICDD uses mixed atmospheric particulate matter collected in an urban area including PAH, nitro-substituted PAHs (nitro-PAHs), polychlorinated biphenyl (PCB) congeners, and chlorinated pesticides in atmospheric particulate material and similar matrices to simulate pollution exposure. The different effects can be measured using:

BBS or BBS+ package

The Bioenergetic Balance Screen (BBS/BBS+) is a performant multiplexed assay developed to measure mitochondrial energy production and central metabolism in a same cell. This test helps you identify anti-pollution formulations that target cell energy and better understand the mechanism of action of your active ingredients or finished products.

The BBS is the multiplexed and integrated measurements, in a same cell, of:

  • Oxygen consumption
  • ATP production
  • Glycolysis level
  • Cell viability
  • TCA cycle turning (BBS+ only)
Mitochondrial dynamics

A module of ICDD's Mitostream® technology, the mitochondrial dynamics package comprises the analysis of:

  • Fusion-fission dynamics
  • Mitochondrial network mass and density
MMP

Examine the mitochondrial membrane potential as an indicator of permeability transition induced by pollutant-induced damages in mature skin.