Anti-aging & mitochondria

Mitochondria reflect cell condition and altered mitochondrial behavior and function have been recognized in age-associated skin alterations.

Mitochondria are particularly involved in 4 key processes of aging:

  • Oxidative stress
  • Inflammation
  • Glycation
  • DNA damage

ICDD's solutions

Human primary senescent cell model

Use our human primary senescent cell model combined with our innovative and performant technologies to evaluate your active ingredients' or finished products' anti-aging efficacy and their ability to reverse age-induced damages.

Banking of human fibroblasts

ICDD banks human fibroblasts from donors of different ages and genders. Mitochondrial behavior signatures have been established to segregate age groups. Let us test your product's ability to reverse an age-induced phenotype.

Which technologies to use

mtDNA content

The mitochondrial DNA content (mtDNA) is a highly relevant image analysis assay and a very good marker of senescence to demonstrate anti-aging properties. It measures:

  • Mitochondrial DNA content
  • Mitochondrial mass
  • Mitochondrial biogenesis
  • Nucleus integrity/morphology
  • Cell viability
BBS or BBS+ package

The Bioenergetic Balance Screen (BBS/BBS+) is a performant multiplexed assay developed to measure mitochondrial energy production and central metabolism in a same cell. This test helps you identify anti-age formulations that target cell energy and better understand the mechanism of action of your active ingredients or finished products.

The BBS is the multiplexed and integrated measurements, in a same cell, of:

  • Oxygen consumption
  • ATP production
  • Glycolysis level
  • Cell viability
  • TCA cycle turning (BBS+ only)
Redox status package

The Redox status package demonstrates anti-oxidant action following physical stress such as IR-, UV-, or heat-exposure.

This package includes the measurement of (any or all):

  • Cellular and mitochondrial radical oxygen species (ROS) production
  • MnSOD and cSOD activities
  • Total Gluthation activity
  • Cellular catalase activity
  • Lipid peroxidation level (TBARs)
  • Protein carbonylation